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Connexions
Présentation
Groupes
Group
Group : Milieu Intérieur
Darragh Duffy
Services
Booking siteCB UTechS proposes various services to the community :
- Training for autonomous use of the equipment
- Assisted use for a selection of technologies
- Advice and technical support
- Development of new of applications and pipelines
- Scientific collaborations
- Access to different data analysis software and project-tailored bioinformatics support for analysis of large-scale data generated by our technologies
Contact : [email protected] for more information.
Training @ Teaching
TRAINING The team provides training for the technologies and data-analysis. The trainings are scheduled on a monthly basis. Theoretical and hands-on training sessions are designed for basic and advanced autonomous users. All trainings are […]
Équipements
Booking siteCB UTechS is an integrated technology platform for multidimensional cell phenotyping & molecular profiling. We propose single-cell cutting-edge technologies and data analysis pipelines. All technologies are bookable through the PPMS online equipment and training reservation site. The equipment is installed in six dedicated laboratories, with a significant part of the technologies situated within BSL2+ (biosafety level 2 +) environment allowing processing of human and infectious material. View a non-exhaustive list of pathogens currently manipulated in the laboratory.
Laboratories and equipment location
Cell phenotyping and sorting (Flow Cytometry Platform)
- – 3 Conventional cytometers (Symphony A5, LSRFortessa, Cytoflex for simultaneous detection of up to 28 parameters
- – Spectral cytometer (ID7000) for indept phenotyping (>40 parameters) and management of autofluorescence
- – Imaging cytometer MARKII (Luminex): spatial quantification of intra- and inter-cellular processes in cells in suspension
- – 7 Cell sorters (2 Astrios, 4 FACSAria Fusion, 1 FACSAria S6): simultaneous sorting of ≤ 6 cell populations, using ≤28 fluorophores in a single panel. 2 sorters available in Bsl2+ environment for sorting of human and/or infectious material.
- – 2 Magnetic-based cell sorters (AutoMACS): pre-enrichment of rare cell populations.
- – MultiMACS: isolation of targeted cell populations from whole blood.
Cell culture and functional assays in Bsl2+ environment (Biomarkers Platform)
- Fully-equipped cell culture facility
- Hypoxia cabinet XVivo (Biospherix): manipulation and cell culture under controlled O2, CO2, N2, temperature and humidity
- Seahorse (Agilent): measuring cellular glycolysis and mitochondrial respiration under controlled O2, CO2, N2, temperature and humidity
- Incucyte S6 (Sartorius): short- and long-term live-content imaging in any conventional cell culture support
- Amaxa nucleofector (Lonza): nucleofection
Multiplex and ultrasensitive protein quantification (immunoassays) (Biomarkers Platform)
- Bioplex 200 (Biorad) coupled to DropArray (Curiox): quantification of ≤ 200 analytes in cell lysis, supernatants or body-fluids with pM sensitivity from reduced sample (5uL) and reagent volumes.
- Simoa HD-x (Quanterix): digital ELISA for quantification of proteins with fM sensitivity
- SP-X (Quanterix): ultrasensitive multiplex protein detection (10-plex)
- Isolight (Isoplexis): single -cell sectretome analysis (32-plex)
RNA/DNA profiling in bulk (Single Cell Platform)
- Nanostring (Nanostring): direct multiplex RNA quantification (800-plex)
- Bioanalyzer (Agilent): quality control of DNA, RNA, & proteins
- Qubit: RNA-quantification
Multi-modal single cell profiling (Single Cell Platform)
- 10xGenomics (3 instruments): single-cell transcriptomics, single-nuclei transcriptomics CITE-Seq, ATAC-Seq, TCR/BCR rearrangement. Available in Bsl2+ confinement for analysis of human and/or infectious cells.
- Spatial transcriptomics (10xGenomics)
- MARS-Seq
- Biomark (Fluidigm): multiplex single cell real-time PCR (96-plex)
Data analysis
- Schnapps –shiny application for analysis of scRNA-Seq data by biologists
- FLowJo (BD) – a tool of choice for analysis of multidimensional flow cytometry data
- Qlucore- user-friendly PCA tool for analysis of high-dimensional data
Seahorse – Cell metabolism • •
Seahorse & Seahorse Mini Simultaneous analysis of mitochondrial respiration and glycolysis in 96-well or 8-well miniplates . With four programmable ports for the addition of liquid media during active assay measurements, it is ideal […]
Incucyte – Live cell imaging • •
Quantitative live cell imaging in a standard incubator using any traditional culture plate/dish. IncuCyte SX5 supports HD phase-contrast, and 2 fluorescence modules (Green/Red, Green/Orange/NIR) and the FRET based Metabolism Optical Module at 4x, 10x […]
BioPlex 200 – protein quantification • •
BioPlex assays are bead-based assays that can be performed in a mixed array (multiplexed). The reader combines two lasers, fluidics, and real-time digital signal processing to distinguish up to 100 different analytes in the […]
Ion Proton & PGM – Next Generation Sequencing • •
Ion semiconductor sequencing for exome, genome & transcriptome high throughput sequencing at reduced cost and complexity. The sequencing run times of <4h and 200bp reads of up to 10Gb (Ion Proton) and <7h and 400 […]
2 • nCounter NanoString – Gene expression profiling • •
nCounter FLEX NanoString A digital color-coded barcode technology for direct multiplexed measurement of gene expression. It allows up to 800 mRNA, miRNA or DNA targets to be simultaneously profiled based on molecular “barcodes” and […]
Fluidigm C1 – single-cell profiling • •
Fluidigm C1 Microfluidic-based technology that enables single cell preparation, RNA extraction, reverse transcription and PCR amplification using just one technology and thus reducing the variability caused by multi-platform technical errors. It supports multiple single-cell […]
BioMark – gene expression profiling • •
BioMark Fluidigm The microfluidics-based technology Biomark, is an automated, high-performance PCR system that processes tens to hundreds of samples in parallel at nanoliter volumes for gene expression, genotyping, copy number variation analysis and digital […]
2100 Bioanalyzer – RNA and DNA quality/quantity • •
Bioanalyzer Chip-based capillary electrophoresis machine for quantification and quality control of DNA, RNA, & proteins. It allows RNA and DNA quality control measurements before downstream experiments.
Qubit 2.0 – DNA/RNA quantification • •
Qubit 2.0 Sensitive quantification of DNA, RNA & proteins using fluorescent dyes. Qubit allows distinguishing between DNA, RNA or free nucleotides and the measurement is not affected by the presence of co-purified contaminants.
ImageStreamX – Imaging cytometry • •
ImageStreamX Integrates flow cytometry (3 lasers) and microscopy (60x magnification) to simultaneously produce 12 images of each cell directly in flow, at rates exceeding 15 000 cells/min. Enables quantification of cell shape changes, internalization, apoptosis, […]
Membres
Milena Hasan
Responsable de Structure
Responsable
Cours
Pasteur Course Advanced immunology
This intensive six-week course, including practical work, lectures, and scientific analysis workshops, covers the fundamental themes of cellular and molecular immunology. For more information and program, please, visit the course webpage.
2025-11-17 09:00:00
2026-01-09 18:00:00
Europe/Paris
Pasteur Course Advanced immunology
This intensive six-week course, including practical work, lectures, and scientific analysis workshops, covers the fundamental themes of cellular and molecular immunology. For more information and program, please, visit the course webpage.
Institut Pasteur, Rue du Docteur Roux, Paris, France
Pasteur Course Single cell gene expression and beyond
The course offers comprehensive theoretical and practical training in all stages of high-throughput single-cell profiling, from sample and library preparation to data analysis. Participants will learn to apply cutting-edge technologies for single-cell multi-omics and […]
2025-06-25 09:00:00
2025-07-02 18:00:00
Europe/Paris
Pasteur Course Single cell gene expression and beyond
The course offers comprehensive theoretical and practical training in all stages of high-throughput single-cell profiling, from sample and library preparation to data analysis. Participants will learn to apply cutting-edge technologies for single-cell multi-omics and […]
Institut Pasteur, Rue du Docteur Roux, Paris, France
Single cell gene expression and beyond
Course Overview The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions. Program This […]
2022-06-30 09:00:00
2022-07-06 18:00:00
Europe/Paris
Single cell gene expression and beyond
Course Overview The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions. Program This […]
28 Rue du Docteur Roux, Paris, France
10th HKU-Pasteur Immunology Course: December 2-15, 2018
‘Quantitative Immunology’ will be the theme of the 10th anniversary of the HKU-Pasteur Immunology Course. We will explore the ongoing transition from reductionist studies, based on the application of genetic approaches in animal models, […]
2018-12-02 09:00:00
2018-12-15 18:00:00
Europe/Paris
10th HKU-Pasteur Immunology Course: December 2-15, 2018
‘Quantitative Immunology’ will be the theme of the 10th anniversary of the HKU-Pasteur Immunology Course. We will explore the ongoing transition from reductionist studies, based on the application of genetic approaches in animal models, […]
HKU-Pasteur Research Centre, Hong Kong
Projets
Projet Transversal
Outils
Financements
Publications
Télécharger-
2025Steroid hormone levels vary with sex, aging, lifestyle, and genetics, Science Advances , 2025, 11 (13), pp.eadu6094. ⟨10.1126/sciadv.adu6094⟩.
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2025IL-23 tunes inflammatory functions of human mucosal-associated invariant T cells., iScience 2025 Feb; 28(2): 111898.
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2024Smoking changes adaptive immunity with persistent effects., Nature 2024 Feb; 626(8000): 827-835.
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2024Beyond 40 fluorescent probes for deep phenotyping of blood mononuclear cells, using spectral technology., Front Immunol 2024 ; 15(): 1285215.
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2023Unveiling Candida albicans intestinal carriage in healthy volunteers: the role of micro- and mycobiota, diet, host genetics and immune response., Gut Microbes 2023 Dec; 15(2): 2287618.
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2023Nix interacts with WIPI2 to induce mitophagy., EMBO J 2023 Nov; 42(22): e113491.
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2023Dissecting human population variation in single-cell responses to SARS-CoV-2, Nature, 2023, 53 (8), pp.e2250307. ⟨10.1038/s41586-023-06422-9⟩.
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2023T cell migration and effector function differences in familial adenomatous polyposis patients with APC gene mutations, Frontiers in Immunology, 2023, 14, pp.1163466. ⟨10.3389/fimmu.2023.1163466⟩.
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2023Single-cell transcriptomic profiling of the mouse cochlea: An atlas for targeted therapies., Proc Natl Acad Sci U S A 2023 Jun; 120(26): e2221744120.
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2023Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans, mBio, 2023, pp.e00870-23. ⟨10.1128/mbio.00870-23⟩.
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+Voir la liste complète de publications
Contact
Phone: 01.40.61.30.27. Email: [email protected] Address 25-28 Rue du Docteur Roux 75015, Paris France